arg 1 Search Results


gene exp arg1 mm00475988 m1  (Thermo Fisher)
99
Thermo Fisher gene exp arg1 mm00475988 m1
MCP enhances T cell activation and modulates macrophage polarization. ( A ) CD44 expression measured by flow cytometry as MFI on OT-I CD8+ T cells activated with 50 ng/mL SIINFEKL, SIIQFEKL, SIITFEKL, or SIIVFEKL with or without 100 µg/mL MCP for 48 hours in vitro. ( B, C, D ) CD25, CD69, PD-1 expression. IL-12β ( E ) and NOS2 ( F ) expression measured by quantitative reverse transcription PCR in bone marrow-derived M0 macrophages polarized to the M1 phenotype with or without 100 µg/mL MCP for 24 hours in vitro. ( G ) <t>Arg1</t> expression in M0 macrophages polarized to the M2 phenotype with or without MCP for 24 hours in vitro. P values determined by Student’s t-test. *p<0.05; **p<0.01. IL, interleukin; MCP, metoclopramide; MFI, mean fluorescence intensity; PD-1, programmed cell death protein-1.
Gene Exp Arg1 Mm00475988 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse  (R&D Systems)
93
R&D Systems mouse
MCP enhances T cell activation and modulates macrophage polarization. ( A ) CD44 expression measured by flow cytometry as MFI on OT-I CD8+ T cells activated with 50 ng/mL SIINFEKL, SIIQFEKL, SIITFEKL, or SIIVFEKL with or without 100 µg/mL MCP for 48 hours in vitro. ( B, C, D ) CD25, CD69, PD-1 expression. IL-12β ( E ) and NOS2 ( F ) expression measured by quantitative reverse transcription PCR in bone marrow-derived M0 macrophages polarized to the M1 phenotype with or without 100 µg/mL MCP for 24 hours in vitro. ( G ) <t>Arg1</t> expression in M0 macrophages polarized to the M2 phenotype with or without MCP for 24 hours in vitro. P values determined by Student’s t-test. *p<0.05; **p<0.01. IL, interleukin; MCP, metoclopramide; MFI, mean fluorescence intensity; PD-1, programmed cell death protein-1.
Mouse, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse arginase 1 arg 1 antibody  (R&D Systems)
93
R&D Systems anti mouse arginase 1 arg 1 antibody
MCP enhances T cell activation and modulates macrophage polarization. ( A ) CD44 expression measured by flow cytometry as MFI on OT-I CD8+ T cells activated with 50 ng/mL SIINFEKL, SIIQFEKL, SIITFEKL, or SIIVFEKL with or without 100 µg/mL MCP for 48 hours in vitro. ( B, C, D ) CD25, CD69, PD-1 expression. IL-12β ( E ) and NOS2 ( F ) expression measured by quantitative reverse transcription PCR in bone marrow-derived M0 macrophages polarized to the M1 phenotype with or without 100 µg/mL MCP for 24 hours in vitro. ( G ) <t>Arg1</t> expression in M0 macrophages polarized to the M2 phenotype with or without MCP for 24 hours in vitro. P values determined by Student’s t-test. *p<0.05; **p<0.01. IL, interleukin; MCP, metoclopramide; MFI, mean fluorescence intensity; PD-1, programmed cell death protein-1.
Anti Mouse Arginase 1 Arg 1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti arginase1 apc  (R&D Systems)
93
R&D Systems anti arginase1 apc
rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and <t>Arg1</t> staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Anti Arginase1 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti arginase 1 arg1 antibody  (R&D Systems)
98
R&D Systems anti arginase 1 arg1 antibody
rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and <t>Arg1</t> staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Anti Arginase 1 Arg1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti arg1  (Proteintech)
96
Proteintech anti arg1
rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and <t>Arg1</t> staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Anti Arg1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arg 1  (Novus Biologicals)
93
Novus Biologicals arg 1
rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and <t>Arg1</t> staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Arg 1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti harginase1 antibody  (R&D Systems)
90
R&D Systems anti harginase1 antibody
rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and <t>Arg1</t> staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Anti Harginase1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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markers arginase 1  (R&D Systems)
93
R&D Systems markers arginase 1
rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and <t>Arg1</t> staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Markers Arginase 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti human arginase 1 arg1 conjugated to percp  (R&D Systems)
93
R&D Systems mouse anti human arginase 1 arg1 conjugated to percp
rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and <t>Arg1</t> staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.
Mouse Anti Human Arginase 1 Arg1 Conjugated To Percp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arginase 1 arg1 liver arginase antibody  (Novus Biologicals)
92
Novus Biologicals arginase 1 arg1 liver arginase antibody

Arginase 1 Arg1 Liver Arginase Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human arg 1 mabs  (R&D Systems)
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R&D Systems anti human arg 1 mabs

Anti Human Arg 1 Mabs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


MCP enhances T cell activation and modulates macrophage polarization. ( A ) CD44 expression measured by flow cytometry as MFI on OT-I CD8+ T cells activated with 50 ng/mL SIINFEKL, SIIQFEKL, SIITFEKL, or SIIVFEKL with or without 100 µg/mL MCP for 48 hours in vitro. ( B, C, D ) CD25, CD69, PD-1 expression. IL-12β ( E ) and NOS2 ( F ) expression measured by quantitative reverse transcription PCR in bone marrow-derived M0 macrophages polarized to the M1 phenotype with or without 100 µg/mL MCP for 24 hours in vitro. ( G ) Arg1 expression in M0 macrophages polarized to the M2 phenotype with or without MCP for 24 hours in vitro. P values determined by Student’s t-test. *p<0.05; **p<0.01. IL, interleukin; MCP, metoclopramide; MFI, mean fluorescence intensity; PD-1, programmed cell death protein-1.

Journal: Journal for Immunotherapy of Cancer

Article Title: Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanoma

doi: 10.1136/jitc-2025-014080

Figure Lengend Snippet: MCP enhances T cell activation and modulates macrophage polarization. ( A ) CD44 expression measured by flow cytometry as MFI on OT-I CD8+ T cells activated with 50 ng/mL SIINFEKL, SIIQFEKL, SIITFEKL, or SIIVFEKL with or without 100 µg/mL MCP for 48 hours in vitro. ( B, C, D ) CD25, CD69, PD-1 expression. IL-12β ( E ) and NOS2 ( F ) expression measured by quantitative reverse transcription PCR in bone marrow-derived M0 macrophages polarized to the M1 phenotype with or without 100 µg/mL MCP for 24 hours in vitro. ( G ) Arg1 expression in M0 macrophages polarized to the M2 phenotype with or without MCP for 24 hours in vitro. P values determined by Student’s t-test. *p<0.05; **p<0.01. IL, interleukin; MCP, metoclopramide; MFI, mean fluorescence intensity; PD-1, programmed cell death protein-1.

Article Snippet: Primers include murine Gapdh (Mm99999915_g1), B2m (Mm00437762_m1), Arg1 (Mm00475988_m1), Nos2 (Mm00440502_m1), Il-12β (Mm01288989_m1), and PRL (Mm00599950_m1).

Techniques: Activation Assay, Expressing, Flow Cytometry, In Vitro, Reverse Transcription, Derivative Assay, Fluorescence

rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and Arg1 staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.

Journal: iScience

Article Title: βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer

doi: 10.1016/j.isci.2022.103758

Figure Lengend Snippet: rβig-h3-structured collagen I fibers modulate the phenotype and function of macrophages (A) Representative photographs of immunofluorescence staining of pancreata from three-months-old KC mice stained for βig-h3 (green) and CD206 (red). Nuclear counterstaining in DAPI (blue). (B) Representative photographs of βig-h3, F4/80, CD206, and Arg1 staining on serial section of pancreata from three-months-old KC mice. (C) FACS analysis of BMMCs cultured alone or on collagen I structured in the absence (BMMCs + Col I) or presence of rβig-h3 (BMMCs + Col I rβig-h3 ). Representative data of three independent experiments with three in vitro replicates per group are shown. ∗p<0.05, ∗∗p<0.01. Error bars 50 μm, 25 μm.

Article Snippet: The following monoclonal Abs were used in flow cytometry: anti-F4/80 Pe-Cy7 (clone BM8-Biolegend, 123114) or PerCP Cy5.5 (clone BM8-Biolegend, 123128), anti-MHCII PE (clone M5/114.15.2- eBioscience, 12-5321-81), anti-CD206 BV650 (clone C068C2-Biolegend, 141723), anti-CD86 BV605 (clone GL-1- Biolegend, 105029), anti-CD80 APC-Cy7 (clone 16-10A1-Biolegend, 104729), anti-CSFRI AF488 (clone AFS98- Biolegend, 135511), anti-INOS PE (clone CXNFT, Life Techno, 12-5920-82), anti-Arginase1 APC (RD, IC5868A), anti-TGF-β B421 (clone TW7-16B4-Biolegend, 141408) and anti-TNFα BV605 (clone MP6-XT22-Biolegend, 506329).

Techniques: Immunofluorescence, Staining, Cell Culture, In Vitro

βig-h3 Ab depletion in vivo reprograms macrophage phenotype in the tumor microenvironment (A) Experimental setting. (B) Tumor weight of mice treated in A. (C-F) FACS analysis of the percentages of CD45 + (C), F4/80 + CD45 + (D), CD206 + F4/80 + (E), and Arg1 + F4/80 + (F) cells collected in A. ∗p<0.05, ∗∗p<0.01. Representative of two independent experiments with fivemice per group.

Journal: iScience

Article Title: βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer

doi: 10.1016/j.isci.2022.103758

Figure Lengend Snippet: βig-h3 Ab depletion in vivo reprograms macrophage phenotype in the tumor microenvironment (A) Experimental setting. (B) Tumor weight of mice treated in A. (C-F) FACS analysis of the percentages of CD45 + (C), F4/80 + CD45 + (D), CD206 + F4/80 + (E), and Arg1 + F4/80 + (F) cells collected in A. ∗p<0.05, ∗∗p<0.01. Representative of two independent experiments with fivemice per group.

Article Snippet: The following monoclonal Abs were used in flow cytometry: anti-F4/80 Pe-Cy7 (clone BM8-Biolegend, 123114) or PerCP Cy5.5 (clone BM8-Biolegend, 123128), anti-MHCII PE (clone M5/114.15.2- eBioscience, 12-5321-81), anti-CD206 BV650 (clone C068C2-Biolegend, 141723), anti-CD86 BV605 (clone GL-1- Biolegend, 105029), anti-CD80 APC-Cy7 (clone 16-10A1-Biolegend, 104729), anti-CSFRI AF488 (clone AFS98- Biolegend, 135511), anti-INOS PE (clone CXNFT, Life Techno, 12-5920-82), anti-Arginase1 APC (RD, IC5868A), anti-TGF-β B421 (clone TW7-16B4-Biolegend, 141408) and anti-TNFα BV605 (clone MP6-XT22-Biolegend, 506329).

Techniques: In Vivo

Journal: iScience

Article Title: βig-h3-structured collagen alters macrophage phenotype and function in pancreatic cancer

doi: 10.1016/j.isci.2022.103758

Figure Lengend Snippet:

Article Snippet: The following monoclonal Abs were used in flow cytometry: anti-F4/80 Pe-Cy7 (clone BM8-Biolegend, 123114) or PerCP Cy5.5 (clone BM8-Biolegend, 123128), anti-MHCII PE (clone M5/114.15.2- eBioscience, 12-5321-81), anti-CD206 BV650 (clone C068C2-Biolegend, 141723), anti-CD86 BV605 (clone GL-1- Biolegend, 105029), anti-CD80 APC-Cy7 (clone 16-10A1-Biolegend, 104729), anti-CSFRI AF488 (clone AFS98- Biolegend, 135511), anti-INOS PE (clone CXNFT, Life Techno, 12-5920-82), anti-Arginase1 APC (RD, IC5868A), anti-TGF-β B421 (clone TW7-16B4-Biolegend, 141408) and anti-TNFα BV605 (clone MP6-XT22-Biolegend, 506329).

Techniques: Recombinant, Modification, Plasmid Preparation, Activation Assay, Concentration Assay, Software

Journal: iScience

Article Title: Nanosphere pharmacodynamics improves safety of immunostimulatory cytokine therapy

doi: 10.1016/j.isci.2024.108836

Figure Lengend Snippet:

Article Snippet: Arginase 1/ARG1/liver Arginase Antibody [Alexa Fluor® 405], polyclonal , Novus , NBP1-32731AF405.

Techniques: Recombinant, Staining, Cell Culture, Red Blood Cell Lysis, Enzyme-linked Immunosorbent Assay, Isolation, Multiplex Assay, Software, Sterility